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Effect of incubation temperature on the lytic activities of phage isolates PPpW-3 and PPpW-4
| Incubation temp (°C) | EOPa
|
|
|---|---|---|
| PPpW-3 | PPpW-4 | |
| 10 | 0.9 | 0.9 |
| 15 | 1.1 | 1.3 |
| 20 | 1.0 | 1.4 |
| 25 | 1.0 | 1.0 |
| 30 | 0.9 | <10−9 |
| 35 | NGb | NG |
P. plecoglossicida PTH-9802 infected with phage was incubated at each temperature for 3 days, and the EOP was quantified by calculating the ratio of the PFU obtained at each temperature to those obtained at 25°C.
NG, no growth of bacteria.
