TABLE 4.
Pathogenicities of phage-sensitive parent cells and phage-resistant variants of P. plecoglossicida PTH-9802 for ayua
| Strain injected | Dose (CFU/fish) | % Mortality (no. dead/no. tested) |
|---|---|---|
| Parent PTH-9802 | 3.9 × 103 | 100 (10/10) |
| 102 | 100 (10/10) | |
| 101 | 80 (8/10) | |
| 100 | 0 (0/9) | |
| Variant R1 | 2.4 × 104 | 0 (0/10) |
| 103 | 0 (0/10) | |
| 102 | 0 (0/10) | |
| Variant R2 | 4.0 × 104 | 0 (0/10) |
| 103 | 0 (0/10) | |
| 102 | 0 (0/10) | |
| Variant R3 | 1.6 × 104 | 0 (0/10) |
| 103 | 0 (0/10) | |
| 102 | 0 (0/10) | |
| Variant R4 | 1.5 × 104 | 0 (0/10) |
| 103 | 0 (0/10) | |
| 102 | 0 (0/10) |
a
Fish were injected intramuscularly with bacteria and were cultivated at 20°C and observed for 2 weeks. Variants R1, R2, and R3 were resistant to both phage PPpW-3 and phage PPpW-4; variant R4 was resistant only to phage PPpW-4.