TABLE 6.
Kinetics of P. plecoglossicida and phage administered to ayua
| Time after inoculation (h) | Fish no. |
P. plecoglossicida count (log10 CFU g−1) in fish fed:
|
Phage count (log10 PFU g−1) in fish fed:
|
||
|---|---|---|---|---|---|
| Bacteria alone | Bacteria and phage | Phage alone | Bacteria and phage | ||
| 0 | 1 | <2 | <2 | <2 | <2 |
| 2 | <2 | <2 | <2 | <2 | |
| 3 | <2 | <2 | <2 | <2 | |
| 3 | 1 | <2 | <2 | 5.0 | <2 |
| 2 | 3.5 | <2 | 4.9 | 3.1 | |
| 3 | <2 | 4.8 | 5.7 | 4.8 | |
| 12 | 1 | 5.3 | <2 | 3.8 | 3.1 |
| 2 | 4.8 | <2 | 3.1 | 3.9 | |
| 3 | 4.0 | <2 | <2 | 3.9 | |
| 24 | 1 | 4.0 | <2 | <2 | <2 |
| 2 | 4.9 | <2 | <2 | 3.5 | |
| 3 | 6.3 | <2 | <2 | <2 | |
| 48 | 1 | 4.9 | <2 | <2 | <2 |
| 2 | 4.2 | <2 | <2 | <2 | |
| 3 | 4.7 | <2 | <2 | <2 | |
| 72 | 1 | 4.3 | <2 | <2 | <2 |
| 2 | 4.1 | <2 | <2 | <2 | |
| 3 | 4.2 | <2 | <2 | <2 | |
| 120 | 1 | 4.3 | —b | — | — |
| 2 | 4.1 | — | — | — | |
| 3 | 4.2 | — | — | — | |
| 168 | 1 | 4.2 | — | — | — |
| 2 | 4.2 | — | — | — | |
| 3 | 3.9 | — | — | — | |
Three groups of fish (average weight, 2.4 g) were fed bacterium (PTH-9802)-impregnated pellets, pellets impregnated with a mixture of phages PPpW-3 and PPpW-4, or phage-impregnated pellets after bacterial challenge (water temperature, 22°C).
—, not done.