Skip to main content
. 2022 Jun 15;17(6):e0270005. doi: 10.1371/journal.pone.0270005

Fig 1. Schematic representation of the experimental setting and body weight variation.

Fig 1

(A) Thirty C57Bl/6 male mice were randomized in 6 groups to receive saline, double oropharyngeal administration (day 0 and 4) of 10 or 15 μg of BLM, and triple OA (day 0, 2, 4) of 5, 6 or 7.5 μg of BLM. Micro-CT (μ-CT) was performed at days 7, 14, 21 and 28. Animals were euthanized at day 28 to collect BALF and lungs for histology. Body weight was reported as percentage of variation compared to the baseline (day 0) for saline, double OA (B) and triple OA (C) groups. Data were shown as mean ± SEM. Statistical analysis was performed via two-way ANOVA followed by Tukey’s test * p<0.05, ** p<0.01 vs. saline; # p<0.05, ## p<0.01 comparing 10 μg vs. 15 μg BLM and 5 μg vs. 6 μg BLM; $$ p<0.01 comparing 7.5 μg vs. 6 μg BLM). N = 5 per group.