Table 4. Antimicrobial activity of treated and untreated Reactive black 5 samples against bacteria.

Reactive Black 5
Tested Bacterial strains	Antimicrobial effect of treated vs. untreated dye samples (zone of inhibition in mm±Std. Dev.)
	0 day (untreated samples)			6 days (treated samples)
	50 ppm	150 ppm	250 ppm	50 ppm	150ppm	250ppm
Pseudomonas aurignosa	8.0±0.5 h	11.0±0.2 e	13.0±0.5 c	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i
E.coli	9.0±0.3 g	11.0±0.2 e	14.0±0.2 b	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i
Staphyloccous aureus	8.0±0.2 h	12.0±0.3 d	13.0±0.3 c	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i
Positive Control	10.0±0.5 f	15.0±0.5 a	15.0±0.3 a	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i
Negative Control	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i	0.0±0.0 i

Positive control used is antibiotic (ampicillin) and negative control used is water. Different alphabets show the significant differences (p < 0.05) between the treatments.