Skip to main content
. 2022 Jun 3;18(6):e1010599. doi: 10.1371/journal.ppat.1010599

Fig 3. RNA binding activity of SAFA is critical for increasing the accessibility of anti-viral chromatin.

Fig 3

(A) Models depicting the ATAC-seq and RNA-seq assay in Flag-SAFA and Flag-Del-RGG stably expressed SAFA−/− THP-1 cells with VSV infection for 6 hours. (B) Heatmap (log RPKM+1) showing the ATAC-seq signal. (C) GO term enrichment analysis of genes significantly affected by RGG domain depletion in ATAC-seq. (D) Genome browser views of ATAC-seq signal for the indicated genes. (E) Violin graph (log RPKM) showing ISGs affected by RGG domain depletion in ATAC-seq. (F) Transcription factor enrichment analysis of ATAC-seq. (G) Heatmap (log FPKM+1) showing RNA-seq signal for the indicated genes. ***p < 0.001, ****p < 0.0001 (Student’s t test; E). The cells were infected by VSV at 0.1 MOI. Data were pooled from two independent experiments (B, C and E-G). Data were representative of two independent experiments (D).