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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: FASEB J. 2020 Mar 26;34(5):6129–6146. doi: 10.1096/fj.201902982R

Figure 8.

Figure 8.

Precursor ion chromatogram of phosphorylated 13-mer synthetic peptides centralized around S151 of Arhgef2, S151 of Crtc1 and S559 of Lipe. The synthetic peptides were phosphorylated following an in vitro phosphorylation reaction in presence of various human recombinant SNF1-subfamily of kinases; (A) MARK3, (B) SIK2, (C) NUAK2, (D) MELK. The phosphorylation occurs predominantly at the target site of the respective peptides as determined by the chromatogram area of site-specific product ions. All SNF1-subfamily enzymes can phosphorylate the three selected phosphorylation sites on Arhgef2 (S151), Crtc1 (S151) and Lipe (S559) to different extent under in vitro condition.