Table 1.

Vasopressin responsive phosphorylation sites in PKA-null cells

Accession	Gene Symbol	Site(s)	Protein Name	Centralized Sequence	Median Log2(dDAVP/ Vehicle)	−log10 (Pfp)
P59729	Rin3	S411	Ras and Rab interactor 3	GISRTAS*LNLPPQ	0.86	3.52
Q8R4H2	Arhgef12	S41	Rho guanine nucleotide exchange factor 12	KVERSSS*HDFDPT	0.79	3.28
Q8C1B1	Camsap2	S439	Calmodulin-regulated spectrin-associated protein 2	GIIRSVS*NEGLTL	0.75	3.16
Q9Z1E4	Gys1	S711¶	Glycogen [starch] synthase, muscle	GSKRSNS*VDTGPS	0.70	2.98
P45481	Crebbp	S120	CREB-binding protein	LGAMGKS*PLNQGD	0.59	2.63
Q9D279	Misp	S364	Mitotic interactor and substrate of PLK1	GLQRSLS*SDCILS	0.59	2.67
Q9ERG2	Strn3	S202	Striatin-3	RSQRVRS*LLGLSN	0.58	2.95
Q3UHI0	Ccser2	S222	Serine-rich coiled-coil domain-containing protein 2	KMVRSQS*FSHSIQ	0.58	2.66
P19001	Krt19	S59	Keratin, type I cytoskeletal 19	VTSSSGS*YGGVRG	0.52	2.47
Q68ED7	Crtc1	S151	CREB-regulated transcription coactivator 1	SWRRTNS*DSALHQ	0.49	2.24
Q3TWF6	Wdr70	S641	WD repeat-containing protein 70	MFAQVES*DDEESK	0.49	2.75
A6H8H2	Dennd4c	S1321	DENN domain-containing protein 4C	YKDRSTS*LSALVR	0.48	1.98
Q9CY58	Serbp1	S203	Plasminogen activator inhibitor 1 RNA-binding protein	SGSDRSS*FSHYSG	0.48	2.18
Q8BG32	Psmd11	S14	26S proteasome non-ATPase regulatory subunit 11	EFQRAQS*LLSTDR	0.48	1.97
Q3B7Z2	Osbp	S377	Oxysterol-binding protein 1	GHKRTGS*NISGAS	0.39	2.48
Q60875	Arhgef2	S163	Rho guanine nucleotide exchange factor 2	GHFNDES*PLGLRQ	0.39	2.11
Q60875	Arhgef2	S151	Rho guanine nucleotide exchange factor 2	SLAKSVS*TTNIAG	0.39	2.31
Q9ESE1	Lrba	T1011	Lipopolysaccharide-responsive and beige-like anchor protein	SNTELQT*HDMSSD	0.37	2.44
Q6GYP7	Ralgapa1	S772	RalGTPase-activating protein subunit alpha-1	TRVRHFS*QSEDTG	0.33	2.62
Q9ESE1	Lrba	S1000	Lipopolysaccharide-responsive and beige-like anchor protein	SIGRASS*IDSASN	0.30	2.75
Q9Z1W9	Stk39	S383	STE20/SPS1-related proline-alanine-rich protein kinase	RRVPGSS*GHLHKT	0.29	2.60
P54310	Lipe	S559	Hormone-sensitive lipase	SMRRSVS*EAALAQ	0.25	3.34
P99027	Rplp2	S79	60S acidic ribosomal protein P2	VSAAPGS*AAPAAG	−0.18	1.33
Q8BTM8	Flna	S968	Filamin-A	SVGVSPS*LDLSKI	−0.20	2.98
Q9JKB3	Ybx3	S52	Y-box-binding protein 3	AALLAGS*PGGDAA	−0.20	2.75
Q62074	Prkci	T411	Protein kinase C iota type	PGDTTST*FCGTPN	−0.34	2.39
Q62074	Prkci	T415	Protein kinase C iota type	TSTFCGT*PNYIAP	−0.35	2.32
Q9D1C9	Rrp7a	S99	Ribosomal RNA-processing protein 7 homolog A	KPDLAES*PTEPKS	−0.35	2.12
Q8C0T5	Sipa1l1	S1626	Signal-induced proliferation-associated 1-like protein 1	HGEFSAS*DSSLTD	−0.45	1.99
Q9Z2H5	Epb41l1	S378	Band 4.1-like protein 1	TFFRLVS*PEPPPK	−0.45	2.39
P99027	Rplp2	S86	60S acidic ribosomal protein P2	AAPAAGS*APAAAE	−0.59	3.65
P39447	Tjp1	S280	Tight junction protein ZO-1	LSDSIHS*ANASER	−0.65	2.25
Q3UHX2	Pdap1	S60¶	28 kDa heat- and acid-stable phosphoprotein	EKKSLDS*DESEDE	−0.83	1.25
P11276	Fn1	S285¶	Fibronectin	ASAGSGS*FTDVRT	−0.84	3.67
Q99J36	Thumpd1	S88	THUMP domain-containing protein 1	DQQPSGS*EGEDDD	−0.94	4.60
P09405	Ncl	S145	Nucleolin	NAKKEDS*DEDEDE	−1.64	16.00
Q3THK3	Gtf2f1	S311	General transcription factor IIF subunit 1	SESSEES*EEEKPP	−2.46	15.60

^¶These sites have redundant quantification evidence showing similar changes (e.g. PKA-null and PKA-intact cells, site containing peptides with or without missed cleavage, and HCD-EThcD pairs). The MS1 spectra of monophosphopeptides containing these sites can be found in Supplemental Figure 3. Eleven of the increased phosphorylation sites are putative SNF1-subfamily targets based on the presence of an (R/K)-(T/S)-X-p(S/T) motif with nonpolar amino acid in position −5 and/or position +4 relative to the phosphorylated amino acid (underlined amino acids).