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Digestion (2 h) of bacterial cellulose by Cel6A (▴) and Cel7A (■) from H. insolens in 50 mM sodium phosphate buffer at pH 6.5 and 37°C. The percentage of degradation is represented as a function of the ratio of enzyme to substrate (nanomoles of cellulase per milligram of cellulose). A quantity of 1.85 nmol of cellulase/mg of cellulose (arrow) was used for all further experiments. See the text for details.
