FIG. 3.

Synergistic degradation of bacterial cellulose by various ratios of Cel6A and Cel7A from H. insolens at a constant total enzyme load of 1.85 nmol of cellulase/mg of cellulose. The dotted line represents the theoretical hydrolysis value expected for a noncooperative degradation. Assays were performed with 50 mM sodium phosphate buffer at pH 6.5 and 37°C for 1 h. The Cel6A-Cel7A mixture (62.5%:37.5%) indicated with an arrow was chosen for subsequent experiments. See the text for details.