Extended Data Fig. 10. Biochemical characterization of MRM3 and GTPBP10 knockout cells.

a, CRISPR/Cas9 mediated targeted knockout of mS37, MRM3 and GTPBP10. The schematics illustrates the guide RNA design for targeted exon in each corresponding gene. Sequencing results and alignment with the wild type validates the knockouts indicated by bp deletions (gray dotted lines) and insertions. b, Metabolic labeling with [³⁵S]-methionine of mitochondrial translation products in wild type (WT) HEK293T, MRM3 and GTPBP10 knockouts and MRM3- and GTPBP10 rescue cells. Coomassie blue stained gel (bottom panel) is shown as a loading control. c, Mitoribosomal sedimentation on 10-30% sucrose gradient for WT HEK293T, MRM3 and GTPBP10 knockout cells. In panels b and c, representative gels from three independent biological experiments are shown. For source data, see Supplementary Fig. 6.