Fig. 1. Domains of mouse anti-Müllerian hormone receptor II (AMHR2) and their gene expression in mouse epithelial ovarian carcinomas (EOCs). (A) Depiction of the different domains of the full-length sequence of the mouse AMHR2 protein indicating the signal peptide, the extracellular domain (AMHR2-ED), the transmembrane domain, and the cytoplasmic domain (AMHR2-CD) with amino acid numbers (upper panel), and the recombinant AMHR2-ED used as immunogen in the current study (lower panel) with a FLAG tag at the N-terminus and a 6xHis tag at the C-terminus. (B) Quantitative reverse transcription-polymerase chain reaction analysis of the ID8 and mouse ovarian carcinoma (MOVCAR) EOC cell lines indicating that both cell lines express AMHR2-ED and AMHR2-CD domains, but the expression of each AMHR2 domain in MOVCAR cells was consistently about twice that of the expression levels measured in ID8 cells. Error bars indicate ±standard deviation.