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. 2022 May 23;45(6):376–387. doi: 10.14348/molcells.2022.2221

Fig. 2. MiR-153-3p was highly expressed in LUAD.

Fig. 2

(A) qRT-PCR analysis of miR-153-3p expression in LUSC cell lines (YTMLC-90, NCI-H266, and SK-MES-1) and LUAD cell lines (NCI-H1993, SW1271 and NCI-H522). (B) TEM of EVs derived from LUAD cell lines: EVs/ NCI-H1993, EVs/SW1271, and EVs/NCI-H522. (C) Nanoparticle tracking analysis of EVs/NCI-H1993, EVs/SW1271, and EVs/NCI-H522. (D) Western blot analysis of exosome markers (CD63, TSG101, Alix, and Hsp90) and non-exosome markers (GRP94 and Cytochrome) in LUAD cells lysate and separated EVs. (E) qRT-PCR analysis of miR-153-3p expression in EVs derived from LUSC cell lines (YTMLC-90, NCI-H266, and SK-MES-1) and LUAD cell lines (NCI-H1993, SW1271, and NCI-H522). (F) Heat map of the significant differentially expressed miRNAs extracted from selected lung carcinomas cell populations (small cell lung carcinoma, adenocarcinoma, and squamous cell carcinoma) compared with adjacent normal tissues (GSE74190). The data in the figures are represented the mean ± SD. **P < 0.01.