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. 2022 May 30;45(6):388–402. doi: 10.14348/molcells.2022.2232

Fig. 5. Levels of invasion-related functional proteins in HBL52 cells following manipulation of KLF4 and IMAT1 expression.

Fig. 5

(A) RT-qPCR assay. Relative levels of KLF4, IMAT1, hsa-miR22-3p, and Snai1 were calculated using the 2-ΔCt method. (B) Western blotting. Quantitative analyses of the KLF4, Snai1, and invasion-related functional proteins (MMP2/9, α-SMA, vimentin, and E-cadherin) in HBL52 cells in various groups after 72 h of infection. *P < 0.05, **P < 0.01, vs HBL52 group; #P < 0.05, ##P < 0.01, vs HBL52 + Lv-KLF4 group (t-test). The tests were carried out in biological triplicates, and data are expressed as the mean ± SD.