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. 2022 Jun 15;5:586. doi: 10.1038/s42003-022-03530-6

Fig. 6. Polyamine depletion mediates global histone hypoacetylation and progesterone biosynthesis.

Fig. 6

a H3K27Ac enrichment near transcription start sites is decreased in male PHTs following DFMO treatment. Orange line represents vehicle and blue line represents DFMO treated PHTs. b Unsupervised principal component analysis of H3K27Ac binding to genomic regions in vehicle and DFMO treated male and female PHTs. c Overlap of DBR annotated genes in H3K27Ac ChIP-seq analysis and DEG in RNA-seq analysis. d Custom views of differentially acetylated region in vehicle and DFMO treated male PHTs. Polyamine depletion decreased e HSD3B1 mRNA and f progesterone secretion in male PHTs. ChIP-seq experiments performed in N = 6 male PHTs and N = 5 female PHTs; progesterone secretion measured in N = 5 male PHTs and N = 5 female PHTs. Male and female samples are coloured blue and red respectively.