Table 1.

Number, frequencies (%) and 95% confidence intervals (CI) of sheep tested positive for D. nodosus (aprV2+ and/or aprB2+) and positive for virulent D. nodosus (aprV2+) by real-time PCR in the different levels of each source of variation.

Source of variation	Level	Number of sheep	D. nodosus-positive sheep (aprV2+ and/or aprB2+)			Virulent D. nodosus-positive sheep (aprV2 +)
			Frequency	CI		Frequency	CI
Region	North	4238	42.21	40.72	43.72	36.86	35.40	38.33
	East	3463	38.78	37.15	40.43	34.33	32.75	35.94
	South	1596	52.44	49.96	54.92	52.32	49.83	54.79
Months	Jan–Feb	1204	49.83	46.97	52.70	47.76	44.90	50.62
	Mar–Apr	1858	32.62	30.49	34.80	26.10	24.12	28.16
	May–Jun	1898	50.21	47.94	52.48	49.68	47.41	51.96
	Jul–Aug	999	37.84	34.82	40.93	34.93	31.98	37.98
	Sep–Oct	1399	24.73	22.49	27.08	21.44	19.32	23.69
	Nov–Dec	1939	56.01	53.77	58.23	48.17	45.92	50.42
Herdbook	Yes	4885	31.34	30.04	32.66	24.01	22.82	25.24
	No	4412	55.26	53.78	56.73	54.69	53.21	56.17
Year	2019	3369	54.70	53.01	56.40	49.27	47.57	50.98
	2020	5928	35.86	34.64	37.10	32.49	31.30	33.70
Sex	Male	1375	27.71	25.36	30.16	25.02	22.75	27.40
	Female	7922	45.29	44.19	46.40	40.92	39.84	42.02
Flock size	< 41	762	30.62	28.12	33.20	29.15	26.69	31.71
	41–100	1724	44.95	42.68	47.24	41.85	39.60	44.12
	101–200	1922	31.58	28.89	34.37	30.09	27.44	32.84
	201–500	2525	31.04	28.80	33.35	19.85	17.94	21.88
	> 500	2364	55.52	53.82	57.21	52.34	50.63	54.04
Antibiotics (3–10)	Yes	495	35.96	31.73	40.36	27.27	23.39	31.43
	No	237	29.11	23.41	35.35	29.11	23.41	35.35
	No Response	8565	43.46	42.40	44.51	39.49	38.45	40.53
Footbaths (3–10)	Yes	276	32.97	27.45	38.86	27.54	22.35	33.21
	No	462	40.69	36.18	45.33	33.55	29.25	38.06
	No Response	8559	43.11	42.06	44.17	39.20	38.16	40.24
Footrot (12)	Yes	4535	59.40	57.96	60.84	57.95	56.50	59.39
	No	3346	23.43	22.00	24.90	18.47	17.17	19.83
	No Response	1416	34.68	32.19	37.22	24.01	21.81	26.32