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Analysis of inhibition of CODH-catalyzed TNT degradation. The degradation of TNT with enzyme treated with CN (▴) or left untreated (●) is shown. CODH (1.8 μg) was treated with 10 eq/αβ KCN for about 0.5 h and then assayed as described in the text in 2 ml of assay buffer (lacking cyanide) with 220 μM TNT and 1 atm of CO. An equivalent sample not treated with KCN was also assayed.
