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. 2022 Jun 2;13:887125. doi: 10.3389/fimmu.2022.887125

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Copyright © 2022 Deng, Tian, Song, An and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA in vitro transcription strategy. The strategy for mRNA preparation consists of template preparation, in vitro transcription, 5′ cap addition, 3′ poly(A) tailing, and purification.