Senescence-associated β-galactosidase staining buffer

Reagent	Final concentration	Amount
Solution A (potassium ferrocyanide)	10 μL/mL	500 μL
Solution B (potassium ferricyanide)	10 μL/mL	500 μL
Solution C (citric acid-sodium phosphate)	880 μL/mL	44 mL
X-gal solution	100 μL/mL	5 mL
Total	n/a	50 mL

Storage temperature: −20°C; maximum time for storage: 12 months.