REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GFP	Invitrogen	Cat# A11122; RRID: AB_221569
Mouse monoclonal anti-Sox2	Abcam	Cat# ab79351; RRID: AB_10710406
Goat anti-Rabbit IgY (H+L) CF®488	Biotium	Cat# 20012; RRID: AB_10559670
Goat anti-Mouse IgY (H+L) CF®568	Biotium	Cat# 20101; RRID: AB_10559187
Bacterial and virus strains
Escherichia coli (E. coli) DH5α competent cells	TransGen Biotech	Cat# CD201-01
Chemicals, peptides, and recombinant proteins
Sodium pentobarbital	Sigma-Aldrich	Cat# 52944-66-8
Sucrose	Sigma-Aldrich	Cat# 57-50-1
Mounting medium	Invitrogen	Cat# P36961
Proteinase K	cwbiotech	Cat# CW2584M
PCR 2× Mix buffer	cwbiotech	Cat# CW0690L
PBS	ZSGB-BIO	Cat# ZLI-9061
Depilatory cream	Veet	N/A
Bovine serum albumin (BSA)	Sigma-Aldrich	Cat# 1933
Normal goat serum (NGS)	ZSGB-BIO	Cat# ZLI-9021
Paraformaldehyde	Solarbio	Cat# P1110
O.C.T. Compound	SAKURA	Cat# 4583
Saline	Solarbio	Cat# IN9000
2×YT medium	Sigma-Aldrich	Cat# Y2377
Fast Green	Sigma-Aldrich	Cat# F7252
Triton X-100	Sigma-Aldrich	Cat# X100
Critical commercial assays
Senescence-associated β-galactosidase staining kit	Beyotime	Cat# C0602
EndoFree Maxi Plasmid Kit V2	TIANGEN	Cat# DP120
Experimental models: Organisms/strains
Mouse: Rack1F/F (C57BL/6, E14.5 and E18.5)	(Zhao et al., 2015)	N/A
Oligonucleotides
Primer used for genotyping: Rack1 loxP-F:	This paper	CGCTGCGCCTCTGGGATCTCA
Primer used for genotyping: Rack1 loxP-R:	This paper	TGGTGTGGCCGACAAATCGCC
Recombinant DNA
pCAG-dCre-GFP	A gift from Dr. Weixiang Guo, Institute of Genetics and Developmental Biology, CAS	N/A
pCAG-Cre-GFP	A gift from Dr. Weixiang Guo, Institute of Genetics and Developmental Biology, CAS	N/A
Software and algorithms
Image J	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
NDP 2.0 view	Hamamatsu	https://www.hamamatsu.com/jp/en/product/type/U12388-01/index.html
GraphPad Prism8	Prism	https://www.graphpad.com/scientific-software/prism/
Olympus FV-1200	Olympus	https://www.olympus-lifescience.com.cn/es/support/downloads/
Olympus Fluoview Ver.4.2b View	Olympus	https://www.olympus-lifescience.com/es/support/downloads/#!dlOpen=%23detail847249651
Adobe Photoshop CC	Adobe	https://www.adobe.com/products/photoshop.html
Other
Electro Square PoratorTM	BTX	ECM 830
Confocal Laser Scanning Microscope	Olympus	FV1200
NanoZoomer Digital Pathology	Hamamatsu	NanoZoomer2.0
Cryomold	Thermo Fisher Scientific	CRYOSTAR NX50
Incubator	Thermo Fisher Scientific	IMC18