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. 2022 Jun 13;3(2):101450. doi: 10.1016/j.xpro.2022.101450

Figure 2.

Figure 2

Principle of infection of C57BL/6J mice with 2000 Trypanosoma brucei parasites

(A) Infection procedure: Begin by thawing a blood stabilate from liquid nitrogen or −80°C storage. Centrifuge the stabilate for 2 min at 800 g and remove all supernatant to remove glycerol. Resuspend in 10 mL of HMI-11 complete media and recover for 24 h at 37°C in 5% CO2. Count parasites using a suitable method (e.g., various automatic counting methods or manually using a Neubauer chamber), and dilute (or concentrate) to have 2000 parasites/200 μL volume. If various mice will be infected, calculate accordingly. Inject 200 μL intraperitoneally into C57BL/6J mice.

(B and C) How should T. brucei look in morphology? (B) shows T. brucei immediately after thawing a stabilate from liquid nitrogen or −80°C storage (left image shows bright field image; right image shows TdTomato fluorescence). (C) shows T. brucei after 24 h of culture in HMI11 media (left image shows bright field image; right image shows TdTomato fluorescence). Scale bar: 5 μm.