Figure 3.

Order of surgeries and IVM. Schematic showing the order we suggest to follow if multiple surgeries will be performed in the same animal

This is further explained in Table 1. Surgeries with low risk include the brain (unless a full craniotomy is performed) (1), the interscapular brown adipose tissue (isc-BAT) (2), the kidneys (3), the lymph node (4), and the gonadal white adipose tissue (5). Surgeries with medium risk include the spleen (6), pancreas (7) and liver (8). The surgeries with the highest risk include the heart (9) and the lungs (10). Created with BioRender.com.