HMI-11 medium

Reagent	Final concentration	Amount
IMDM with 25 mM HEPES and L-Glutamine		880 mL
Heat inactivated Fetal Bovine Serum	10%	100 mL
Hypoxanthine (100×)	1/100	10 mL
Bathocuproinedisulfonic acid (1000×)	1/1000	1 mL
L-cysteine (100×, 18.2 mg/mL)	1/100	10 mL
Pyruvic acid (98%)	100 mg/L	69.2 μL
2-mercaptoethanol (100 mM)	1/500	2 mL
Total		1 L

Store at 4°C. Stocks of FBS should be kept at −20°C. Upon thawing and addition of FBS to HMI-11 media, use within 4 weeks.