REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental models: Organisms/strains
Mouse (mus musculus): C57BL/6, males/females, 6–10 weeks	Charles River	Strain code: 027
Mouse (mus musculus):: CD36−/−, males/females, 6–10 weeks	The Jackson Laboratory	Stock code: 019006
Experimental models: Cell lines
T. brucei TdTom/Ty1/RFLuc	Calvo-Alvarez et al., 2018	https://pubmed.ncbi.nlm.nih.gov/29339220/
T. brucei PAD1::GFP	Zimmermann et al., 2017	https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006324
Chemicals, peptides, and recombinant proteins
Rompun 2% (xylazine) (16 mg/kg)	Bayer	NA
Imalgene 1000 (ketamine) (120 mg/kg)	Merial	NA
Buprex or Budale (Buprenorphine 0.3 mg/mL) (100 μL / 15–30 g every 8–12 h)	Richter pharma	NA
Carboxfluorescein diacetate succinimidyl ester (CFDA-SE) – 20 μM	Invitrogen	Cat# V12883
7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridine-2-one) succinimidyl ester (DDAO-SE)- 10 μM	Invitrogen	Cat# C34564
70 kDa FITC-Dextran (dilution: 500 mg/kg)	Sigma -ldrich	CAS 60842-46-8
Hoechst 33342 (dilution: 40 μg/kg)	Sigma-Aldrich	CAS 875756-97-1
HMI-11 culture media	See Table 1	See Table 1
IMDM with 25 mM HEPES and L-Glutamine	Invitrogen	Cat# E15-819
Heat inactivated Fetal Bovine Serum	PAA	Cat# A15-104
Hypoxanthine	Thermo Fisher Scientific	Cat# A11481-06
Bathocuproinedisulfonic acid	Sigma-Aldrich	Cat# 146625-5G
L-cysteine	Sigma-Aldrich	CAS 52-90-4
Pyruvic acid	Sigma-Aldrich	CAS 127-17-3
2-mercaptoethanol	Sigma-Aldrich	CAS 60-24-2
RPMI-1640 media	Thermo Fisher Scientific	Cat #11875101
Phosphate Buffered Saline	Thermo Fisher Scientific	Cat# 20012027
Heparin sodium	Sigma-Aldrich	CAS 9041-08-1
Antibodies
Alexa Fluor 647 anti-mouse CD36 clone HM36. Isotype Armenian Hamster IgG 0.5 mg/mL (20 μg/ 30 g body weight)	BioLegend	Cat# 102610
Alexa Fluor 647 rat anti-mouse CD62P (P-selectin) clone RB40.34 0.2 mg/mL (20 μg/ 30 g body weight)	BD Pharmingen	Cat# 563674
Alexa Fluor 647 anti-mouse CD102 (ICAM2) clone 3C4 (MIC2/4). Isotype Rat IgG2a,k (20 μg/ 30 g body weight)	BioLegend	Cat# 105612
Alexa Fluor 647 anti-mouse CD54 (ICAM1) clone YN1/1.7.4. Isotype Rat IgG2b,k (20 μg/ 30 g body weight)	BioLegend	Cat# 116114
Alexa Fluor 647 anti-mouse CD31 (PECAM1) clone 390. Isotype Rat IgG2a,k (20 μg/ 30 g body weight)	BioLegend	Cat# 102416
Mouse CD106/VCAM-1 FITC conjugate (50 μg/ 30 g body weight)	Invitrogen	Cat# RMCD10601
Purified Rat Anti-mouse CD102 (20 μg/ 30 g body weight)	BD Pharmingen	Cat#553326
Blocking antibody RB40.34 against CD62-P (20 μg/ 30 g body weight)	BD Biosciences	Cat# 553742
Blocking antibody P2H3 against CD62-E (20 μg/ 30 g body weight)	eBioscience	Cat# 14-0627-82
Blocking antibody CBR 1C2/2 against CD102 (20 μg/ 30 g body weight)	eBioscience	Cat# BMS109
Blocking antibody YN1/1.7.4 against CD45 (20 μg/ 30 g body weight)	eBioscience	Cat#16-0541-85
Blocking antibody against CD31 (20 μg/ 30 g body weight)	Abcam	Cat#ab32457
Blocking antibody against CD36 (185-1G2) (20 μg/ 30 g body weight)	Thermo Fisher Scientific	Cat# MAS-14112
Blocking antibody Mouse IgG1 K isotype control (P3.6.2.8.1) (20 μg/ 30 g body weight)	eBioscience, Thermo Fisher Scientific	Cat# 16-4714-82
Blocking antibody Mouse IgG2 K isotype control (eBM2a) (20 μg/ 30 g body weight)	eBioscience, Thermo Fisher Scientific	Cat# 14-4724-82
Critical commercial assays
AlexaFluor 647 Conjugation Kit	Abcam	Cat# ab269823
Software and algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
SlideBook 6.0.22	Intelligent Imaging Innovations	https://www.intelligent-imaging.com/slidebook
ZEN 2.6 (blue edition)	Carl Zeiss Imaging	https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
GraphPad Prism 9	GraphPad Software Inc	https://www.graphpad.com/scientific-software/prism/
TopSolid	TopSolid	https://www.topsolid.com/products/topsoliddesign.htm
Imaris 9.7	Oxford Instruments	https://imaris.oxinst.com/versions/9-7
BioRender	BioRender	https://biorender.com
Other
Dumoxel 3C Tweezers	Electron Microscopy Services	Cat# 0103-3C-PO
Dumoxel Style1 Tweezers	Electron Microscopy Services	Cat# 0103-1-PO
S&T JF5TC SuperGrip Forceps Straight	Interfocus	Cat# 00632-11
Standard Pattern Forceps Straight 13 cm	Interfocus	Cat# 11000-13
Crile Hemostat Straight	Interfocus	Cat# 13004-14
Sharpening Stone Square	Interfocus	Cat# 29008-01
Fine Scissors Straight Large Loops 10 cm	Interfocus	Cat# 14040-10
Fine Scissors ToughCut Straight 9 cm	Interfocus	Cat# 14058-09
Vannas Spring Scissors Straight 3 mm Cutting Edge	Interfocus	Cat# 15000-00
Scissors Straight BluntBlunt 12 cm	Interfocus	Cat# 14000-12
MayoHegar Needle Holder	Interfocus	Cat# 12004-18
Moria Instrument Case	Interfocus	Cat# 20311-21
Surgical scalpel	Securimed	Cat# 2185
Surgical glue	3M VetbondTM/MC tissue adhesive	Cat# 1469SB
Surgical tape	3 M	Cat# 1530-0
Cotton swabs and cotton balls	NA	NA
Various surgical sutures (kit)	Pinnacle Medics	NA
8-0 Polypropylene surgical sutures	Johnson & Johnson	Cat# 8740H
6-0 Polyglactin surgical sutures	Johnson & Johnson	Cat# D5890
4-0 Silk surgical sutures	Johnson & Johnson	Cat# K881H
Cautery pen	AgnThos	59016
Surgical drill	BrainTree scientific	MD-1200 120V
Nitrile examination gloves	Ansell	NA
Ventilator	Ugo Basile	Cat# 28025
Heating lamp	Welch Allyn	Cat# LS-150
Rib retractor	Fine Science Tools	Cat# 17003-03
Animal warmer	Softron	Cat# THC-31
Endotracheal intubation tube	Generated in-house	Maruyama et al., 2021
Insulin Needles	BD	Cat# 326769
Rectangular coverglass (#1.5, 100 mm × 60 mm)	Menzel Gläser	NA
Circular coverglass (#1.5, 12 mm)	Menzel Gläser	NA
3i Marianas SDC microscope	Intelligent Imaging Innovations	SN# 1648
Zeiss Cell Observer SDC microscope	Carl Zeiss microimaging	SN# 3851001637
Zeiss LSM 880	Carl Zeiss microimaging	SN# 2811000267
Zeiss LSM 710	Carl Zeiss microimaging	SN# 2503000400
Multiphoton Microscope Leica TCS SP8 MP	Leica Microsystems	https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8-mp/