FIGURE 1.

Cell cycle timing and regulation of CENP-A. Modified from Mendiratta et al., 2019 . (A) CENP-A and its chaperone HJURP are regulated in a cell cycle–dependent manner. (B) Transcription is regulated in cis by the CHR/CDE motif in their promoter region. The recruitment of the DREAM complex at the CHR is thought to promote transcriptional repression during G1. At the beginning of S phase, the MuvB core of the DREAM complex remains bound to the CHR/CDE while other components dissociate and are replaced by B-MYB. The B-MYB-MuvB (MMB) complex recruits FOXM1 in late S phase. B-MYB is degraded upon phosphorylation, whereas the progressive phosphorylation of FOXM1 leads to its activation in G2/M. (C) CENP-A and HJURP mutually stabilize each other at the protein level. The reciprocal stabilization is affected by posttranslational modifications of CENP-A.