Figure 1.

C3a/C3a-desArg, C4a/C4a-desArg and C5a/C5adesArg levels in human plasma after treatment with B. jararaca venom. Human blood samples containing the peptide GPRP (8 mg/mL) were incubated with increasing concentrations of B. jararaca venom or sterile saline solution for 30, 60 and 120 minutes at 37°C. After plasma collection and dilution, the production of anaphylatoxins C3a/C3a-desArg (1:5000), C4a/C4a-desArg (1:5000) and C5a/C5a-desArg (1:1000) was evaluated by cytometric bead array (CBA). The results are presented as the mean ± SEM from three independent tests. ns, not significant; *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p < 0.0001.