Figure 6.

Production of anaphylatoxins in human blood stimulated with B. jararaca venom in the presence of Cp40. Human blood samples containing GPRP (8 mg/mL) were preincubated with cp40 inhibitor (20 μM) and then incubated with B. jararaca venom (50.0 μg/mL) or sterile saline solution for 60 minutes at 37°C. After plasma collection and dilution, the production of anaphylatoxins C3a/C3a-desArg (1:5000), C4a/C4a-desArg (1:5000) and C5a/C5a-desArg (1:1000) was evaluated by cytometric bead array (CBA). The results are presented as the mean ± SEM from three independent experiments. ns, not significant; *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p < 0.0001.