Figure 7.

Levels of TNF-α and chemokines in human blood stimulated by B. jararaca venom in the presence of Cp40. Human blood samples containing GPRP (8 mg/mL) were preincubated with Cp40 inhibitor (20 μM) and then incubated with B. jararaca venom (50.0 μg/mL) or sterile saline solution for 60 minutes at 37°C. After plasma collection and dilution (1:2), the presence of TNF-α and the chemokines IL-8, MCP-1 and MIG was evaluated by the Cytometric Bead Array (CBA). The results are presented as the mean ± SEM from three independent experiments. ns, not significant; *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0005, ****p < 0.0001.