Figure 4.

Adoptive transfer of γδ T cells aggravated SS in NOD mice. (A) Changes in SG weight and saliva flow rates were measured in NOD/Ltj mice adoptively transferred with γδ T cells. (B) Histological examination on the tissue destruction in lung of NOD/Ltj mice after transferred with γδ T cells or PBS, stained with H&E. (C) The number of Vγ4⁺ T cells and Vγ4⁺ IL-17⁺ T cells in NOD/Ltj mice transferred with γδ T cells was lower than those injected with PBS in the peripheral blood, while the proportion of Vγ4⁺ T cells and Vγ4⁺ L-17⁺ T cells in the peripheral blood was unchanged. (D) The proportion and number of Vγ4⁺ T cells and Vγ4⁺ L-17⁺ T cells in the lung of NOD/Ltj mice with γδ T cell transfer were higher than those in NOD/Ltj mice injected with PBS. (n=10, *, P<0.05; **, P<0.01; ***, P<0.001, ns, no significance). The black arrows indicate lymphocytic infiltration. The 150 µm scale stands for the whole staining image, while 50 µm scale stands for the small staining image with higher magnification to better see the inflammatory infiltration. SS, Sjögren’s syndrome; SG, salivary gland; PBS, phosphate-buffered saline; PB, peripheral blood; NOD, non-obese diabetic; H&E, hematoxylin and eosin.