TABLE 2.

ssODNs for per mutagenesis.

Genotype	ssODN sequence in 5′ to 3′ direction
S45S	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGTCAC​TCC​TCC​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S45A	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​GCG​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S45D	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​GAC​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​A\GCG​ACA​T
S45F	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​TTC​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S45E	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​GAG​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S44A	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​GCG​TCC​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S44D	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​GAC​TCC​GGC​AGC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S47A	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​TCC​GGC​GCG​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S47D	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​TCC​GGC​GAC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S44-45A	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​GCG​GCG​GGC​TTC​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT
S45-47A	ATC​CAT​CCC​CTT​TCC​AGT​GGC​AGC​TCC​AAA​TCC​CGC​CTG​AGC​GGC​AGT​CAC​TCC​GCG​GGC​GCG​AGT​GGC​TAT​GGG​GGC​AAG​CCC​TCG​ACG​CAG​GCC​AGC​AGC​AGC​GAC​AT