TABLE 3.

Molecular screening for per mutagenesis.

Product name	Product size	Forward primer(5′to 3′)	Reverse primer(5′to 3′)
per	1332	GTT​ACC​CCC​ATT​CAA​GGT​CC	CCA​AAA​CGG​GCA​CAG​ATA​CT
Act5-Cas9	771	GAT​AAA​AAT​CTG​CCT​AAC​G	CTT​ATG​CCT​TCC​CAT​TAC​T
Vasa-Cas9Set1	762	GATAAGAACCTGCCCAAC	GCCCATCACTTTCACGA
Vasa-Cas9Set2	761	GCCTACCACGAGAAGTA	AGGATGGGCTTGATGAA
lig4	784	GCCAGCACGATCAAGTTC	GGTCTGCCAGCAGATTG