Table 4.
Details of randomly selected SSR markers (thirteen dinucleotide and two trinucleotide repeats) along with their primer sequence and melting temperature ranging from 57.17°C to 60.99°C.
| ID | SSR type | Unit | SSR | Primer Pairs (5′-3′) | Tm (°C) |
|---|---|---|---|---|---|
| TRINITY_DN252967_c0_g2_i1 | Tri | 10 | (TGT)10 | SP-SSR-F1-TGTTGTTGTTGCTTGTGTTGT | 57.62 |
| SP-SSR-R1-TCATTTTATTTGCACATAAACTGCTT | 57.17 | ||||
| TRINITY_DN510811_c3_g6_i3 | Di | 10 | (TC)10 | SP-SSR-F2-GACACGCACAAACACCCATT | 59.61 |
| SP-SSR-R2-TGACAGAAAAACAGAAAACAGAACA | 58.02 | ||||
| TRINITY_DN473094_c8_g3_i1 | Di | 19 | (GA)19 | SP-SSR-F3-GTCGTCCTCCTATGCACTCG | 59.97 |
| SP-SSR-R3-CCGCGTGCTGGATTAATTGG | 59.97 | ||||
| TRINITY_DN513544_c0_g11_i2 | Di | 10 | (TC)10 | SP-SSR-F4-CTGATGATGTTGCGGGCATG | 59.97 |
| SP-SSR-R4-CTCACTGTTGAGCTGCACAA | 58.69 | ||||
| TRINITY_DN503444_c13_g12_i1 | Di | 17 | (AG)17 | SP-SSR-F5-GGAGAGAGATTGGGCGAGTG | 59.89 |
| SP-SSR-R5-AGGTATTCCCTCCTTCCCCC | 60.02 | ||||
| TRINITY_DN438564_c3_g5_i2 | Di | 12 | (TC)12 | SP-SSR-F6-GGTATGTACCAGTAGCTATGTGT | 57.21 |
| SP-SSR-R6-CCCATTGTCACCACGGGTAT | 59.74 | ||||
| TRINITY_DN456622_c3_g1_i1 | Di | 10 | (GT)10 | SP-SSR-F7-TAGTCAGAGACGGGCATCCA | 60.03 |
| SP-SSR-R7-ACACACTTCCACATGTTTATCTGC | 59.78 | ||||
| TRINITY_DN523626_c2_g1_i3 | Di | 17 | (TC)17 | SP-SSR-F8-TGTCGTGATGCCCAAGTTGT | 60.17 |
| SP-SSR-R8-TGGGTGCCATCCATTGACTC | 60.03 | ||||
| TRINITY_DN511017_c1_g2_i7 | Di | 10 | (AG)10 | SP-SSR-F9-TGTCAGACTTGCTAGGCGAC | 59.75 |
| SP-SSR-R9-TCAAGACCCACATGACACCT | 58.56 | ||||
| TRINITY_DN431119_c0_g4_i2 | Di | 10 | (AG)10 | SP-SSR-F10-GTGTTGGGGAACGTAGCAGA | 59.96 |
| SP-SSR-R10-GCGCTTGGATCGGAATCAAC | 59.97 | ||||
| TRINITY_DN408106_c2_g1_i1 | Di | 10 | (AG)10 | SP-SSR-F11-TCGAAATCGAAAAACATGTCCACA | 59.72 |
| SP-SSR-R11-TGTGCTTTATACTTCGATGTTGTGA | 59.07 | ||||
| TRINITY_DN500214_c8_g1_i2 | Di | 10 | (TC)10 | SP-SSR-F12-ACGAACTGCTTGGGTGAGTT | 59.82 |
| SP-SSR-R12-TTTGTCCCGGCCTTGTTCTT | 60.10 | ||||
| TRINITY_DN459549_c1_g2_i1 | Di | 11 | (TG)11 | SP-SSR-F13- GTTGCGTGCGTGTGTGTG | 60.64 |
| SP-SSR-R13-ACGCCTTCTCTTCCCTCTCT | 59.96 | ||||
| TRINITY_DN517244_c1_g1_i2 | Di | 19 | (AG)19 | SP-SSR-F14-GTTGCATACGAGGAGGGGAC | 60.17 |
| SP-SSR-R14-TCTCCCTCTCCCTCTCCCTC | 60.99 | ||||
| TRINITY_DN17441_c0_g1_i1 | Tri | 13 | (CAA)13 | SP-SSR-F15-GCCATGTGATGCAGCAACAA | 60.03 |
| SP-SSR-R15-GCTGAGCTTGGATGATACTCT | 57.25 |