Table 5.

Differentially Expressed Transcripts (DETs) and primers used for qPCR.

Sr. No.	Transcript ID	Primer Pair	Primer Sequence	Tm (°C)	GC %	Amplicon Size (bp)
1	TRINITY_DN521405_c2_g2_i1 (Avenin-like a4)	SP-QF33	TGCCCTTGCTGCTGTCGCATGA	55.44	59.09	137
		SP-QR33	ACAGATGTGGCAGGCAAGCGGT	57.08	58.33
2	TRINITY_DN483169_c0_g1_i6 (Alpha-amylase inhibitor 0.19)	SP-QF24	AGCCGAGTACGACGCATGGAGCGTT	58.21	60.00	119
		SP-QR24	TGCCATTGCACTGGAGCCTCAGCA	57.21	58.33
3	TRINITY_DN518599_c1_g1_i2 (Aspartic proteinase oryzasin-1)	SP-QF31	TTAAGCTAGCCCGCTTGTGCCA	52.72	54.55	114
		SP-QR31	ACGGCAAACTAGCGAATCCTGCGT	55.07	54.17
4	TRINITY_DN326896_c0_g1_i1 (Elongation factor 1-alpha)	SP-QF10	TGAAGGAGCCCTTTCCCATCTCAGCA	55.92	53.85	122
		SP-QR10	ACACGTAGATTCGGGCAAGTCCACCA	56.02	53.85
5	TRINITY_DN385005_c2_g1_i2 (Zinc transporter 6)	SP-QF12	GTCCCTGCTGTCAGGTGGAGGAATAA	54.07	53.85	125
		SP-QR12	ACGACCGACATGCATCTGAAGTGGA	54.23	52.00
6	TRINITY_DN425597_c0_g2_i2 (heat shock protein 83-like)	SP-QF15	AAGCCAGAAGACAGGAGCGCAGTT	54.72	54.17	148
		SP-QR15	ACATGGCAGCAAAGAAACACCTGGAG	54.01	50.00
7	TRINITY_DN490415_c0_g1_i8 (Alpha-gliadin)	SP-QF25	ATTTCCGCGAACTGGGGCAGTTGT	55.47	54.17	119
		SP-QR25	TCCTTCCAACAGCCTCAGCAGCAA	54.81	54.17
8	TRINITY_DN365152_c1_g1_i1 (Curcuminoid synthase)	SP-QF11	ACGCATTCCGTAGCGCCATTGT	53.39	54.55	143
		SP-QR11	TATATTGTCCAGGATCGGACGCCCT	53.19	52.00
9	TRINITY_DN472370_c0_g2_i1 (Uncharacterized protein)	SP-QF21	AGAGGCCTTGGGGTTGAAACAACCTT	55.02	50.00	146
		SP-QR21	TTCATCCCGCATCGCCAGTTCTGCTT	56.99	53.85
10	TRINITY_DN441674_c0_g2_i1 (Putative uncharacterized protein)	SP-QF17	GTTTGTTTTACGGCGTAGCCTCCCGA	55.24	53.85	143
		SP-QR17	ACTCAAGCCTCGCTTGGTATTGGGCA	56.65	53.85

GC content of the primers was ranged from 50.00 to 60.00% and the size of amplicon was observed to be ranged from 114 to 148.