FIGURE 4.

Enriched environment exposure alters the transcriptome-wide distribution of m⁶A. (A) MeRIP-seq data analysis revealed the sequence motif of m⁶A modification in SH and EE groups. (B) Cumulative distribution of m⁶A abundance (log₂(enrichment folds+1)) in SH (blue line) and EE (red line) groups. p value was calculated using the two-sided Wilcoxon and Mann–Whitney test. (C) The distribution pattern of m⁶A modification in the transcriptome of the hippocampal tissues of SH and EE mice. m⁶A tag was significantly enriched around the stop codon of mRNAs. (D) Venn diagram illustrating the specific and overlapped m⁶A peaks in the transcriptome of the hippocampal tissues of SH and EE mice. (E) Scatterplot showing the differential m⁶A enrichment of the overlapped peaks between SH and EE groups, including 2021 hyper-methylated peaks (red dots) and 123 hypo-methylated peaks (blue dots) in the EE group.