Figure 1.

Effects of 2′-fucosyllactose (2′-FL) on fecal microbiota in a pH-controlled single-batch fermentation. (A) Relative abundance of Blautia in the fecal microbiota before and after fermentation in YCFA_2FL (n = 7). (B) Composition of the fecal microbiota for each participant after fermentation in YCFA_2FL. The relative abundances of the top 20 species-level taxa are shown; others are grouped into the “other” category. (C) TLC of 2′-FL utilization in fecal cultures for each participant after fecal fermentation in YCFA_2FL. Responder: participants with increased Blautia after fermentation in YCFA_2FL. Non-responder: participants without increased Blautia after fermentation in YCFA_2FL. 2′-FL, 2′-fucosyllactose and YCFA_2FL, YCFA medium supplemented with 1.0% (w/v) 2′-fucosyllactose as the sole carbon source.