TABLE 4.
Estimation of h2a of resistance from laboratory selection of the Melaka population of P. xylostella with Cry1Ac, Cry1Ab, B. thuringiensis subsp. kurstaki, and B. thuringiensis subsp. aizawai (F4 to F8) and reselection of Cry1Ac-SEL population with Cry1Ac (F9 to F13)
| Strain | Estimate of mean response/generationb
|
pd | Estimate of mean selection difference/generation
|
h2h | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Initial LC50 | Final LC50 | Rc | ie | Initial slope | Final slope | ςpf | Sg | |||
| Cry1Ac-SEL | 0.573 (−0.241) | >20 (>1.30)j | 0.308 | 25 | 1.271 | 1.33 | 1.45 | 0.719 | 0.913 | 0.34i |
| Cry1Ac-SEL F15 | 0.573 (−0.241) | 293 (2.47) | 0.301 | 21 | 1.372 | 1.33 | 2.30 | 0.551 | 0.756 | 0.40 |
| Cry1Ab-SEL | 0.509 (−0.293) | 2.13 (0.327) | 0.124 | 34 | 1.078 | 1.32 | 3.51 | 0.414 | 0.446 | 0.28 |
| B. thuringiensis subsp. kurstaki-SEL | 2.183 (0.339) | 6.02 (0.78) | 0.088 | 34 | 1.078 | 0.89 | 3.81 | 0.426 | 0.459 | 0.19 |
| B. thuringiensis subsp. aizawai-SEL | 0.356 (−0.449) | 0.68 (−0.17) | 0.056 | 41 | 0.948 | 4.50 | 2.56 | 0.283 | 0.268 | 0.21 |
Reference 52.
Initial and final LC50s were determined at F3 and F9, respectively (for Cry1Ac-SEL F15, initial and final LC50s were determined at F3 and F15, respectively).
Response to selection (R) = log (final LC50) − log (initial LC50)/n.
Percentage of the population with values above the selection threshold (percentage surviving selection).
Intensity of selection (11).
Phenotypic deviation [0.5 (initial slope + final slope)]−1.
Selection differential (difference in mean phenotype between selected parents and entire parental generation, s = iςp).
h2 = R/S.
Approximately calculated, as LC50 is approximated (Table 3).
Log of LC50s.