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. 2021 Oct 28;17(2):750–762. doi: 10.1016/j.jds.2021.10.003

Figure 4.

Figure 4

NAC promoted osteogenic differentiation and increased the reduced glutathione in PDLSCs stimulated by cyclic mechanical stress. (A) The mRNA levels assay of COL1, RUNX2 and OPN with NAC were performed using real-time PCR. (B) The protein levels assay of COL1, RUNX2 and OPN with NAC were performed. (C, D) The ALP enzyme activity (ALP staining as well as ALP activity tests) increased with NAC in PDLSCs (the scale bar represented 100 and 200 μm). (E) The up-regulated level of reduced glutathione (GSH) and the GSH/oxidized glutathione (GSSG) ratio in PDLSCs with NAC were observed under cyclic mechanical stretch. ∗P < 0.05 versus the control group, respectively; ns, not significant.