Table 1.
The primer sequences used in this study. COL1, collagen type 1; RUNX2, runt-related transcription factor 2; OPN, osteopontin; Nrf2, nuclear factor erythroid-2-related factor-2; HO-1, heme oxygenase-1; NQO1, NADPH dehydrogenase quinone 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
| Genes | Primer | Sequence (5′-3′) |
|---|---|---|
| COL1 | Forward primer (5′-3′) | 5′-TTTGGATGGTGCCAAGGGAG-3′ |
| Reverse primer (5′-3′) | 5′-CACCATCATTTCCACGAGCA-3′ | |
| RUNX2 | Forward primer (5′-3′) | 5′-CACTGGCGCTGCAACAAGA-3′ |
| Reverse primer (5′-3′) | 5′-CATTCCGGAGCTCAGCAGAATA-3′ | |
| OPN | Forward primer (5′-3′) | 5′-TCTCAGAAGCAGAATCTCCTAG-3′ |
| Reverse primer (5′-3′) | 5′-TCTACATCATCAGAGTCGTTCG-3′ | |
| Nrf2 | Forward primer (5′-3′) | 5′-ATCCATTCCTGAGTTACAGTGTCTT-3′ |
| Reverse primer (5′-3′) | 5′-TGTCAGTTTGGCTTCTGGACT-3′ | |
| HO-1 | Forward primer (5′-3′) | 5′-TCTTGGCTGGCTTCCTTACC-3′ |
| Reverse primer (5′-3′) | 5′-GGATGTGCTTTTCGTTGGGG-3′ | |
| NQO1 | Forward primer (5′-3′) | 5′-CTGGTTTGAGCGAGTGTTCA-3′ |
| Reverse primer (5′-3′) | 5′-AGTACATGGAGCCACTGCCA-3′ | |
| GAPDH | Forward primer (5′-3′) | 5′-AGAAGGCTGGGGCTCATTTG-3′ |
| Reverse primer (5′-3′) | 5′-AGGGGCCATCCACAGTCTTC-3′ |
COL1, collagen type 1; RUNX2, runt-related transcription factor 2; OPN, osteopontin; Nrf2, nuclear factor erythroid-2-related factor-2; HO-1, heme oxygenase-1; NQO1, NADPH dehydrogenase quinone 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.