TABLE 1.
Radioactivity incorporated into the benzyl alcohol, benzaldehyde, and benzoic acid produced by B. adusta after l-[U-14C]phenylalanine was addeda
Day of harvest | % of radioactivity inb:
|
||||
---|---|---|---|---|---|
Residual l-phenylalanine | Benzyl alcohol | Benzaldehyde | Benzoic acid | Estimated 14CO2c | |
6 | 26.2 ± 1.3 | 39 ± 2 | 13 ± 0.7 | 3.2 ± 0.2 | 18.6 ± 1 |
8 | 4.3 ± 0.2 | 14 ± 1 | 17.7 ± 0.9 | 1.1 ± 0.05 | 62.9 ± 3 |
l-[U-14C]phenylalanine was added at the time of inoculation.
Radioactivity is expressed as the percentage of the total radioactivity added initially (8.29 μCi/ml).
The estimated amount of 14CO2 was calculated by assuming that aryl metabolites and l-phenylalanine could be completely degraded to carbon dioxide.