TABLE 5.
Effect of cycloheximide on the concentrations of aryl metabolites, extracellular and intracellular enzymatic activities, l-phenylalanine consumption, and mycelial dry weight in B. adustaa
| Concn of inhibitor (mg/liter) | Initial l-phenylalanine concn (mg/liter) | Residual l-phenyl-alanine concn (mg/liter) | Mycelial dry wt (g/liter) | Concn (mg/liter) of:
|
Enzyme activities (U/liter)
|
Enzyme sp act (U/g)
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Benzyl alcohol | Benzaldehyde | Benzoic acid | AAO | LiP | MnP | Laccase | AAO | LiP | PAL | AAD | AADD | Transaminase | MnP | Laccase | ||||
| 0 | 3,100 | 72 ± 12 | 5.54 ± 0.1 | 484 ± 26 | 71 ± 4 | 7 ± 2 | 7.4 ± 2 | 7 ± 2.6 | NDb | ND | 8.75 ± 1 | 37 ± 6 | 25 ± 0.4 | 91 ± 10 | 12 ± 0.8 | ND | ND | ND |
| 10c | 3,100 | 3,000 | 6.3 ± 1.2 | 17 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
a
Activities were determined after 10 days of cultivation.
b
ND, not detected.
c
The inhibitor was added at the time of inoculation.