TABLE 1.
Reference species and EMBL accession no. | No. of isolates | Sequence position relative to the 5′ end of the ITS5 primer
|
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
106 | 128 | 145 | 147 | 150 | 179 | 180 | 181 | 199 | 202 | 211 | 212 | 213 | ||
P. roquefortiX82358 | A | A | T | T | A | – | C | C | A | C | A | A | C | |
48 | • | • | • | • | • | – | Nb | • | • | • | • | • | • | |
2 | G | • | C | C | G | C | • | • | T | T | T | G | A | |
P. paneumX82360 | A | C | C | C | G | – | C | C | A | A | T | G | A | |
2 | • | • | • | • | • | – | • | • | • | • | • | • | • | |
P. carneumX82359 | A | A | T | C | A | – | T | C | A | C | A | A | C | |
0 | • | • | • | • | • | – | • | • | • | • | • | • | • |
Sequence comparison of relevant positions from the ITS1 region of previously published reference isolates (2) and 52 strains isolated from feed and tentatively identified as P. roqueforti by microscopy. Dashes (–) indicate deletions and bullets (•) indicate identical nucleotides.
In two cases, position 180 was T and in one case A, while the remaining 45 positions had a C residue.