MD simulation steps along with the simulation time and temperature for each step.

Stage	Step description	Simulation time (ns)
(1) Equilibrium simulation	(a1) Shortening (melting) of the lipid tails (lipid tail minimization)	2
	(b1) Minimization and equilibration with protein constrained	2
	(c1) Equilibration with protein released	2
	(d1) Production run	100
(2) Steered MD simulation	(a2) Pulling the potassium ion with constant velocity	32
	(b2) Pulling the chloride ion with constant velocity	32
(3) Non-eqSrium simulation by inducing voltage gradient	(a3) Applying external electric field	15