. 2022 May 28;94(23):8156–8163. doi: 10.1021/acs.analchem.1c05206

Table 1. Kinetic and Equilibrium Affinity Binding Constants of Affimers and Nanobodies Derived from SPR Data^a.

binding protein	target	k_a ± SE	k_d ± SE	K_d ± SE
		(M^–1 s^–1) ×10⁴	(s^–1) ×10^–4	(nM)
Affimer 4	GDH	4.4 ± 0.8	8.1 ± 0.1	19 ± 3.2
Affimer 18	TxB	14 ± 3	38 ± 11	26 ± 2.6
Affimer 45	TxB	7.7 ± 1.2	9.4 ± 0.3	13 ± 2.4
Nanobody E3	TxB	10 ± 1	2.5 ± 0.2	2.5 ± 0.04
Nanobody 7F	TxB	2.8 ± 0.5	9.0 ± 0.1	33 ± 6.1

Data are from duplicate (Affimer 4, Affimer 45, and nanobody 7F) or triplicate (nanobody E3) analyte titrations on one chip. Data for Affimer 18 are from three analyte titrations across two chips. Standard errors from the mean are shown.

Table 1. Kinetic and Equilibrium Affinity Binding Constants of Affimers and Nanobodies Derived from SPR Dataa.

Table 1. Kinetic and Equilibrium Affinity Binding Constants of Affimers and Nanobodies Derived from SPR Data^a.