Table 2. Sensitivity (LoD), Accuracy (% Recovery), and Precision (% CV) of TxB and GDH Assays, as Determined from Raw Bioluminescence (RLU; Figures 4 and S11C,D).
intra-assay | interassay | |
---|---|---|
TxB | ||
sensitivity (LoD) | 44 fM | 190 fM |
quantifiable range | 0.1–1000 pM | 1–100 pM |
% recovery | 88–105% | 99–101% |
% CV | 3–25%a | 11–20% |
GDH | ||
sensitivity (LoD) | 4.5 pM | 14 pM |
quantifiable range | 0.01–10 nM | 0.1–10 nM |
% recovery | 100% | 101–102% |
% CV | 1–10% | 19–24%a |
%CV precision metrics > 20% only at the limit of quantification.