Table 1.
Microscopic Analysis of the Adenomas in Tamoxifen-Treated Mice
| Genotype | Mouse ID | Ileum | Colon | ||
|---|---|---|---|---|---|
| # lesions a | Total score b | # lesions a | Total score b | ||
| 1A. Histologic adenoma quantification | |||||
| ApcW/FCdx2ERT2-Cre/Adh1b1+/+ | AA01 | 0 | 3 | ||
| AA04 | 0 | 1 | |||
| AA09 | 0 | 1 | |||
| Apc W/F Cdx2 ERT2-Cre /Aldh1b1 −/− | AA02 | 4 | 1 | ||
| AA05 | 2 | 3 | |||
| AA07 | 1 | 0 | |||
| 1B. p53 IHC quantification | |||||
| Apc W/F Cdx2 ERT2-Cre /Adh1b1 +/+ | AA01 | 0 | 0 | 3 | 105 |
| AA04 | 0 | 0 | 1 | 0 | |
| AA09 | 0 | 0 | 1 | 5 | |
| Apc W/F Cdx2 ERT2-Cre /Aldh1b1 −/− | AA02 | 4 | 16 | 1 | 0 |
| AA05 | 2 | 2 | 3 | 2 | |
| AA07 | 1 | 270 | 0 | 0 | |
| 1C. β-catenin IHC Quantification | |||||
| Apc W/F Cdx2 ERT2-Cre /Adh1b1 +/+ | AA01 | 0 | 0 | 3 | 780 |
| AA04 | 0 | 0 | 1 | 200 | |
| AA09 | 0 | 0 | 1 | 240 | |
| Apc W/F Cdx2 ERT2-Cre /Aldh1b1 −/− | AA02 | 4 | 730 | 1 | 5 |
| AA05 | 2 | 260 | 3 | 670 | |
| AA07 | 1 | 270 | 0 | 0 | |
a
The number of histologically-identifiable adenomas in ileum or colon
b
Total score = (percentage of cells that label positive the immunofluorescent antibody) × (relative intensity of IHC fluorescence signal)