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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: Structure. 2022 Apr 8;30(6):851–861.e5. doi: 10.1016/j.str.2022.03.010

Figure 4: His377 and His378 stabilize the CTT against the flavin pocket in the dark.

Figure 4:

(A) PDS-based spin-spin distance distributions between a flavin radical and a C-terminal nitroxide moiety appended to the CTT (red) by site-enzymatic ligation of a spin-labeled peptide. Distance distributions were obtained upon light-excitation (440 nm, 23.5 mW) with the nitroxide-labeled WT CRY (purple, 4 nm broad distance, undocked CTT) and the L405E/C416N mutant mimicking the dark-state (red, 3 nm narrow distance, docked CTT). (B) Representative distance distributions obtained after restraining to a one or two-state model of Gaussian function. ASQ forming variants were restrained to one-component (the WT parent, purple), while the NSQ forming variants were restrained to two components (mixture of docked (red)/undocked (purple) states).