TABLE 1.
Oligonucleotides used for genomic walking-PCR and amplification of DNA coding for various domain combinations of T. maritima FjSS3B.1 xynB and xynC
| Oligonucleotide | Gene | Sequence | Site |
|---|---|---|---|
| XYNBFULLF | xynB | 5′-GTCTCTCCATATGAAACAGTTCTTGTCCTG | NdeI |
| XYNBFULLR | xynB | 5′-GGAAATCTGGATCCTCACTTGATGAGCCTG | BamHI |
| XYNBCATF | xynB | 5′-AAAGCTCGCCATGGGTCCGGAAGAAGAGATACCCG | NcoI |
| XYNBCATR | xynB | 5′-TACCACAGGAGCTCATTCGGAGATCCTGCTTTCT | SacI |
| XYNCFULLF | xynC | 5′-TGTTTCCATGGGGTCTCTTCTGAGACTGTG | NcoI |
| XYNCFULLR | xynC | 5′-CGGGGAATTCTTTCATTTCAGGAGT | EcoRI |
| XYNCCATF | xynC | 5′-CAAACCGCCCATGGCGTTCGAAGAGGATGTACCTT | NcoI |
| XYNCCATR | xynC | 5′-CACCACGGGAGCTCACTCTGAAATGGTAGAGGTC | SacI |
| TXYNB3 | xynB | 5′-CCAGAGGCACCGAGGACGGCACTCA | |
| TXYNB4 | xynB | 5′-CTTGAAAATCTCAAAGAGCTGCATC | |
| TXYNB5 | xynB | 5′-ATCTCGGCAGAAGTGGTGTCCACTACT | |
| TXYNB6 | xynB | 5′-AGACGGAGGTCGACAGAGAAGACGTAC | |
| TXYNC1 | xynC | 5′-GAGGGGCTTCCAAGAAACGTGGAGG | |
| TXYNC2 | xynC | 5′-TCTGAAGACGCTGAACAGTTGGGCG |