TABLE 2.

PCR amplification of the first TSD domains of xylanase genes from T. maritima MSB8, T. neapolitana, and T. maritima FjSS3B.1

PCR template DNA	Result with primer combinationd:
	APF-APRa	BPF-BPRb	CPF-CPRc
T. maritima FjSS3B.1	−	+	+
T. neapolitana	−	+	+
T. maritima MSB8	+	−	−
No-DNA control	−	−	−

^aXYNAPF (5′-GGAACAACAGAAGGTGTGGTC) and XYNAPR (5′-ACTTCAGATGAAGCTTCGATG), forward and reverse primers for T. maritima MSB8 xynA, respectively. 

^bXYNBPF (5′-GGGGACCACAGACGGTGCTTC) and XYNBPR (5′-ACTTTTGGAGAAATGGAAATC), forward and reverse primers for T. maritima FjSS3B.1 xynB, respectively. 

^cXYNCPF (5′-AAGGAAGAAGATGGGGTTTCA) and XYNCPR (5′-ACCCTGGAAGTGACCTGAAGT), forward and reverse primers for T. maritima FjSS3B.1 xynC, respectively. 

^d+, PCR product of expected size observed; −, no PCR product observed.