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. 2022 Jun 2;13:905899. doi: 10.3389/fimmu.2022.905899

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Copyright © 2022 Zhou, Wu, Liu, Li, Jin and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relish directly activates the transcription of lncRNA-CR33942. (A) Schematic diagram of Relish motif in the lncRNA-CR33942 promoter region. (B) ChIP-seq visualization of Rel-N-GFP using IGV. (C) ChIP-qPCR was performed in Flag-Rel68 overexpressing S2 cells normalized to control expression levels. The expression levels of Relish (D) and lncRNA-CR33942 (E) in Rel68 overexpressing and Rel68-RNAi flies. (F, G) The dual-luciferase reporter assays were performed to detect the activity of the lncRNA-CR33942 promoter with or without Rel68 overexpression. *P < 0.05; **P < 0.01; ***P < 0.001.