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. 2022 Jun 2;12:878881. doi: 10.3389/fcimb.2022.878881

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Copyright © 2022 Wang, Wang, Liu, Wang, Wang, Xu, Huang and Gao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Performance of the primer-probe sets tested with the RPA-LFS system. (A) Showing the LFS assay results of RPA amplification products before mismatch. (B) Showing the LFS assay results of RPA amplification products after mismatch. (C) Agarose gel results. The name of each primer-probe set is shown above the corresponding strip. NTC strip is the no-template control for the corresponding RPA. The positions of the test and control lines are marked on the right side of the bars. Reactions were performed at 37°C for 30 min. This image represents the results of three independent experiments.